Table 2.
Details of antibodies used
| Method | Detected protein | Primary antibody | Concentration (μg/ml) | Secondary antibody | Concentration (μg/ml) |
|---|---|---|---|---|---|
| IHC | Survivin | pAB AF886 (R&D Systems) | 5 | Goat anti-rabbit immunoglobulin/biotinylated (DAKO) | 4 |
| IHC | Survivin | mAB clone 32.1 (Novus Biologicals) | 6 | Swine anti-mouse immunoglobulin/biotinylated (DAKO) | 4 |
| IHC | Survivin | pAB 500.201 (Novus Biologicals) | 4 | Goat anti-rabbit immunoglobulin/biotinylated (DAKO) | 4 |
| IHC | Survivin | mAB clone 60.11 (Novus Biologicals) | 7 | Swine anti-mouse immunoglobulin/biotinylated (DAKO) | 4 |
| IF | Survivin | pAB AF886 (R&D Systems) | 2 | Red fluorescent dye-labeled anti-rabbit immunoglobulin (Invitrogen) | 1 |
| IF | Survivin | mAB clone 60.11 (Novus Biologicals) | 2 | Red fluorescent dye-labeled anti-mouse immunoglobulin (Invitrogen) | 1 |
| IB | Survivin | pAB AF886 (R&D Systems) | 1 | Swine anti-rabbit immunoglobulins/HRP-conjugated (DAKO) | 0,2 |
| IB | Survivin | mAB clone 60.11 (Novus Biologicals) | 2 | Swine anti-mouse immunoglobulin/HRP-conjugated (DAKO) | 0,2 |
Primary and secondary antibodies used for immunohistochemistry, immunofluorescence, and immunoblotting are listed, with inclusion of sources, purpose, and antibody concentrations. IB - immunoblot, IHC - immunohistochemistry, IF - immunofluorescence;