. Author manuscript; available in PMC: 2011 Apr 13.

Published in final edited form as: Arch Microbiol. 2010 Dec 24;193(3):201–208. doi: 10.1007/s00203-010-0663-8

Table 1.

Plasmids and bacterial strains used in this study.

Plasmid construct/Strain	Description/genotype	Source/Reference
pSP72/PTS	ptsH and ptsI; Amp^R	This study
pHSG396/Mtl	mtlA, mtlD, mtlR; Cm^R	This study
pSP72/mtlA^EC	DH5α mtlA; Amp^R	This study
pSP72/mtlA^O395	V. cholerae O395 mtlA; Amp^R	This study
pSP72/mtlA^PS15	V. cholerae PS15 mtlA; Amp^R	This study
pACD4K/mtlA	Mutagenic plasmid; Cm^R	This study
pAR1219	Helper plasmid; Amp^R	Davanloo et al. (1984)
E. coli HB101	F⁻, lacY1, galK2, (Str^r), xyl-5, mtl-1.	Hanahan (1985)
E. coli DH5α	F⁻ endA1 recA1 galE15 galK16 nupG rpsL ΔlacX74 Φ80lacZΔM15 araD139 Δ(ara,leu)7697 mcrA Δ(mrr⁻hsdRMS- mcrBC) λ⁻	Invitrogen, Carlsbad, CA.
E. coli HB/Mtl	mannitol operon, ptsI, ptsH; Amp^R, Cm^R	This study
Vibrio cholerae O395	Wild-type V. cholerae	ATCC 39541/Ogawa 395
Vibrio cholerae PS15	Wild-type V. cholerae non-O1	This study
V. cholerae O395 mtlA:: Intron	mtlA mutant, Kan^R	This study