Table 2.

Oligonucleotide primers used in this study

Primer	Nucleotide sequences (5′-3′)	Target gene	Product size (bp)
PTS1	gcgggatccactattttcatcgcctatctcct	ptsI and ptsH	2330
PTS2	gcgctcgaggacggtttacgctcaattgctca
ManXh	gcgctcgagcggtgtatttttagttgacccgt	Mannitol operon	7256
ManEco	gcggaattcatcatggcgatggatataaccgg
Mtl-ECF	gcgggatccatgtcatccgatattaagatcaa	E. coli mtlA	1914
Mtl-ECR	gcgctcgagttacttacgacctgccagcagttc
Mtl-VCF	gcgggatccatgatatcatcagacgccaaggt	V. cholerae mtlA	1950
Mtl-VCR	gcgctcgagttatgccgcttggctggtggcca
IBS	aaaaaagcttataattatccttaagagacgaaagcgtgcgcccagatagggtg	Mutagenic primers	-
EBS1d	cagattgtacaaatgtggtgataacagataagtcgaaagcggtaacttacctttctttgt
EBS2	tgaacgcaagtttctaatttcgatttctcttcgatagaggaaagtgtct