Table 1. Sequences of the primers used in the experiment.
Primer | Sequence (5′-3′) | Sequence information |
P1 (forward) | AAGGAGGGCACATCAAACTGG | CfDDC specific primer |
P2 (forward) | CTGTGTGCAGAGAATGCTAACGAG | CfDDC specific primer |
P3 (forward) | AGTCCAGCACCAGGATGAGAAG | CfDDC specific primer |
P4 (reverse) | CTGACTCAGATGCCCGATATTCAC | CfDDC specific primer |
P5 (reverse) | TCGTTAGCATTCTCTGCACACAG | CfDDC specific primer |
P6 (reverse) | CAGGTGCCGACACTTCAAATC | CfDDC specific primer |
P7 (forward) | GGCCACGCGTCGACTAGTACT 17 | Oligo(dT)-adaptor |
P8 (reverse) | GGCCACGCGTCGACTAGTACG 10 | Oligo(dG)-adaptor |
P9 (forward) | TGTTAGCCAGACCGTCAG | Real-time CfDDC primer |
P10 (reverse) | ATTATCTCCTTCTTCGTCCTCC | Real-time CfDDC primer |
P11 (forward) | CAAACAGCAGCCTCCTCGTCAT | Real-time actin primer |
P12 (reverse) | CTGGGCACCTGAACCTTTCGTT | Real-time actin primer |
P13 (forward) | ATGGAGAACATTCACAACCGTAGG | CfDDC recombination primer |
P14 (reverse) | TTAAAAATTAAATATAGGTTCATCGAACGA | CfDDC recombination primer |
M13-47 | CGCCAGGGTTTTCCCAGTCACGAC | pMD18-T vector primer |
RV-M | GAGCGGATAACAATTTCACACAGG | pMD18-T vector primer |
T7 | TAATACGACTCACTATAGGG | pEASY-E1 vector primer |
T7t | GCTAGTTATTGCTCAGCGG | pEASY-E1 vector primer |