Table 1. Oligonucleotide recombination efficiency.

	Gal+ recombinant per 108 viable cells*
Oligo†	galKTYR145UAG‡	galK<>cat-sacB‡
100 (T·C)	3.5 × 105 (26)	8.6 × 104 (13)
101 (A·G)	4.8 × 104 (20)	2.8 × 103 (15)
144 (C·C)	3.1 × 107 (3)	1.2 × 105 (2)
145 (G·G)	1.8 × 104 (3)	2.3 × 103 (2)

^*Strain HME6 has point mutation (galK_TYR145UAG), and HME31 has cat-sacB insertion.

^†The relevant part of the oligo sequences is shown in Fig. 1. The mismatch pair (in parentheses) is created by annealing the oligo at galK_TYR145UAG.

^‡Results are presented as an average from several independent experiments (number in parentheses). The standard deviation from the mean was ≈50%.