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SC motifs inhibit GR activity in trans. CV-1 cells were transfected with vectors for either WT (p6RGR) or SC mutant (p6RGR K297R/K313R) GR (30 ng) and the indicated amounts of vectors for Gal4 DBD fusions to either WT (pG4(SC)2) or mutant (pG4(SCmut)2) SC motifs of GR. The reporter plasmids used were pΔ(Gal)2(TAT)3-Luc (A), pΔ(Gal)2(TAT)1-Luc (B), pΔ(Gal)1(TAT)3-Luc (C), and pΔ(Gal)1(TAT)1-Luc (D).
