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Northern and Western analyses of BMV RNAs and proteins from WT BY4743 yeast and selected deletion strains with reduced BMV-directed Rluc expression. Each strain was transformed with pB12 to express 1a and 2a and with pCUP1B3 to express WT RNA3. Percent of WT sgRNA4 accumulation and Rluc expression are indicated. Northern blots of yeast ACT1 and ADH1 mRNAs are shown for comparison. Although not listed in Table 1, SCP160 was included due to its membrane association.
