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. 2011 Apr 13;6(4):e18738. doi: 10.1371/journal.pone.0018738

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Chang et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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The Teflon® culture dish and the ITO-deposited glass plates for culturing the neural stem cells are connected to a biphasic current stimulator. Each well is connected in a shunt electrical configuration, forming a 6-well culture system. (A) Photo of the culture chamber. (B) Schematic diagram of cross section of the culture chamber. (C) 3D view of the setup. (D) Side view of the setup.