Figure 5. The effects of BEC on the neuronal differentiation during 4 days of differentiation.

After 4 days of electrical stimulation at 100 Hz with a magnitude of 1.33, 4 or 8 µA/cm² and duration of 50 or 200 µs, Tuj1 immunopositive neurons were counted for all differentiated cells. (A) Experimental scheme for the electrical stimulation during the differentiation. (B) Left panel: The differentiated NSCs were immunostained with anti-Tuj1 (red) antibodies and counterstained with DAPI (blue). Right panel: Counts of Tuj1 immunopositive neurons. DAPI nuclear staining is for the total numbers of neurons. Data represent mean ± S.E.M. of n = 15–20. ***, p≤0.001 by One-Way ANOVA: Tukey's HSD Post Hoc test. Scale bar = 20 µm.