Fig. 2.

nebula mutation causes perturbations in biochemical signaling. (A) Calcineurin activity determined from extracts of CS, nla¹/+, and nla¹. Calcineurin activity in nla¹ increased by 40%. (B) PKA activity in nla¹ is ≈50% of the CS flies. (A and B) n = 3 experiments done in triplicate (*, P < 0.05). (C) Western blot analysis with anti-pCREB antibody shows that the level of pCREB in nla¹ is significantly reduced. Equal intensity bands seen by Coomassie blue staining of the SDS protein gel confirm equal loading. (D) Quantitative real-time RT-PCR results. d-jun transcript level in nla¹ is ≈30% lower than in the CS flies whether total RNA from whole flies or adult heads was used. n = 4 independent experiments in triplicate. Values represent mean ± SEM.