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. 2011 Mar 31;7(3):333–346. doi: 10.7150/ijbs.7.333

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© Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited.

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Fig 1 — Purification and identification of the Gh-rTDH^WT and Gh-rTDH^mut proteins. (A) Coomassie blue-stained SDS-PAGE of Gh-rTDH^WT and Gh-rTDH^mut proteins with standards. (B) Immunoblot analysis of Gh-rTDH^WT and various Gh-rTDH^mut proteins with antiserum against Gh-rTDH yielded a single band. (C) Non-denaturing PAGE of Gh-rTDH^WT and Gh-rTDH^mut proteins with standards. (D) Hemolytic activity detected when the Gh-rTDH^WT and Gh-rTDH^mut proteins were embedded in a blood agar plate. Lane 1: Phenyl Sepharose 6 Fast Flow purified Gh-rTDH^WT; lane 2: Gh-rTDH^Y53H; lane 3: Gh-rTDH^T59I; lane 4: Gh-rTDH^S63T; lane 5: Gh-rTDH^Y53H/T59I; lane 6: Gh-rTDH^Y53H/S63T; lane 7: Gh-rTDH^T59I/S63T; lane 8: Gh-rTDH^{Y53H/T59I/S63T}.