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. 2011 Mar 31;7(3):333–346. doi: 10.7150/ijbs.7.333

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© Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited.

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Fig 7 — Effect of Congo red on the Gh-rTDH^WT protein. (A) Dose-dependent inhibition of Congo red on the Gh-rTDH^WT hemolysis. Various concentrations of Congo red were pre-incubated with 1 M Gh-rTDH^WT for 30 min, subsequently incubated with human 4% (v/v) erythrocytes for 30 min, and the viability of the cells were determined by the hemolytic activity assay. The data are means ± S.D. from at least three independent experiments. (B) Absorbance spectra of a Congo red solution in the absence or presence of native Gh-rTDH^WT and Gh-rTDH^WT fibrils, respectively. The α-helix rich apo-Mb was used as a negative control. The difference spectrum is obtained by subtracting the Congo red spectrum in the absence of Gh-rTDH^WT fibrils from the corrected Congo red spectrum in the presence of Gh-rTDH^WT fibrils. (C) Representative transmission electron microscopic image of the Gh-rTDH^WT fibrils form after heat treatment.

Fig 7 — Effect of Congo red on the Gh-rTDH^WT protein. (A) Dose-dependent inhibition of Congo red on the Gh-rTDH^WT hemolysis. Various concentrations of Congo red were pre-incubated with 1 M Gh-rTDH^WT for 30 min, subsequently incubated with human 4% (v/v) erythrocytes for 30 min, and the viability of the cells were determined by the hemolytic activity assay. The data are means ± S.D. from at least three independent experiments. (B) Absorbance spectra of a Congo red solution in the absence or presence of native Gh-rTDH^WT and Gh-rTDH^WT fibrils, respectively. The α-helix rich apo-Mb was used as a negative control. The difference spectrum is obtained by subtracting the Congo red spectrum in the absence of Gh-rTDH^WT fibrils from the corrected Congo red spectrum in the presence of Gh-rTDH^WT fibrils. (C) Representative transmission electron microscopic image of the Gh-rTDH^WT fibrils form after heat treatment.