Figure 1.

SOCC activity was examined in JOE and golli KO OPCs. A, Application of either caffeine (Caff) (2 mm) or Tg (100 μm) to JOE and golli KO OPCs elicited robust increases in the fura-2 ratio that were dependent on the presence of external Ca²⁺. The graphs shows the average amplitude calculated from the responding cells, expressed as percentage of change of the emission intensities. Values are expressed as mean ± SEM of at least four independent experiments (n > 400 cells for each condition). *p < 0.05, **p < 0.01 versus respective control. B, Fura-2-loaded JOE and golli KO OPCs were first pretreated with Tg in the presence of zero Ca²⁺ medium and then reexposed to a 2 mm Ca²⁺-containing medium to trigger Ca²⁺ influx via SOCC. Fura-2 ratios in selected cells are plotted with respect to the time of stimulation. The times of addition of Tg- and Ca²⁺-containing external solutions are indicated by the horizontal bars.