Figure 9.

A, Confocal microscopy analysis of JOE OPCs reveal high TRPC1 expression in certain sites along the processes and soma of the cells. Scale bar, 25 μm. B, JOE OPCs were immunostained for golli (red) and TRPC1 (green). Merged images show areas of obvious colocalization and/or juxtaposition (arrows). Note that golli fluorescence is predominantly concentrated in multiple high-intensity patches along the processes, and TRPC1 is found closely associated with these sites of high golli concentration (arrows). Scale bar, 15 μm. C, Time-lapse confocal microscopy of OPC process overexpressing golli–GFP during SOC influx. Peaks in local amplitude of Ca²⁺ uptake were found at several sites along the process. The close interrelationship between golli and Ca²⁺ influx sites can be clearly seen, with golli–GFP being closely surrounded by high levels of fura-2 fluorescence (arrows). D, Correlation analysis of the patterns of local peak Ca²⁺ amplitudes and golli–GFP in the same OPC process shown in C. Local peak Ca²⁺ amplitudes after reexposure to Ca²⁺-containing medium (120 s) (red line) along the process are shown compared with golli–GFP fluorescence measurement (green line) in the same cellular sections.