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. Author manuscript; available in PMC: 2012 Jun 1.
Published in final edited form as: Biotechnol Bioeng. 2011 Feb 24;108(6):1360–1371. doi: 10.1002/bit.23069

Table I.

Oligonucleotide primers utilizing in this study. All sequences are 5’ → 3’ and restriction sites are denoted with an underline

Name Sequence (5’→3’)
BamHI_prpE_for GGGGGATCCATGTCTTTTAGCGAATTTTATCAGCGTTC
HindIII_prpE_rev GGGAAGCTTACCTACGGTTCAGGTCC
NdeI_atoC_for GGGCATATGACTGCTATTAATCGCATCC
XhoI_atoC_rev GGGCTCGAGTTATACATCCGCCGGATCG
NdeI_sbm_for GGGCATATGTCTAACGTGCAGGAGTG
XhoI_sbm_rev GGGGTCGAGTTAATCATGATGCTGGCTTATCAG
pKD13_operon_for AATACCCTCATTTTGATTGCGTTTTACGGAGCAAATAATGATTCCGGGGATCCGTCGACC
pKD13_operon_rev ATTGCTGAAGATCGTGACGGGACGAGTCATTAACCCAGCATGTAGGCTGGAGCTGCTTCG
k2 CGGTGCCCTGAATGAACTGC
ver_operon_rev CGCCCAGCCAGTTGAGTTCA