Figure 1.
Representative transverse histological sections of the detrusor muscle of the bladder. A> Sham control intramural ganglia stained with H&E. B> A serial section of the same ganglia stained for neurofilament (NF) 200. C> Low power micrograph of NF stained nerve bundle (arrow) in detrusor muscle wall. D> Higher power of area indicated with arrow in C. Similar photomicrographs are shown for a root repair animal (F–I), genitofemoral nerve transfer (GF-NT) animal (J–M), and a coccygeal nerve transfer (CG-NT) animal (N–Q). E> A negative control section showing a nerve bundle (n) in which the primary anti-neurofilament antibody was not included. Abbreviations: n -nerve bundle, ct – connective tissue, m – muscle bundle. Scale bars in C,H, L and P are 100 μm; the remainder are 10 μm.
