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. Author manuscript; available in PMC: 2012 Apr 1.
Published in final edited form as: Neurourol Urodyn. 2011 Jan 20;30(4):599–605. doi: 10.1002/nau.21042

Figure 2.

Figure 2

Representative transverse histological sections of the detrusor muscle showing DiI or PGP9.5 labeled axons, and DAPI staining, a general nuclear stain. A> Sham operated control dog in which only a DiI-labeled larger nerve bundle is seen (arrow). B> DiI and DAPI labeling in same field as A showing no detrusor muscle bundles (M) receiving DiI labeled axons directly. C> PGP9.5 labeling in same field showing smaller axons on detrusor muscles (arrowheads) that are not DiI labeled and the larger DiI labeled nerve bundle shown in A and B (arrow). D> Root repair animal showing both DiI labeled nerve bundles (arrow) and DiI labeled smaller axons in association with detrusor muscle bundles (arrowheads). E> DiI and DAPI labeling of same field as D. F> Same field showing PGP9.5+ axons in same locations as the smaller DiI labeled axons on detrusor muscles (arrowheads) and the larger DiI labeled nerve bundle (arrow). G> GF-NT animal showing both DiI labeled nerve bundle (arrows) and DiI labeled smaller axons in association with detrusor muscle bundles (arrowheads). H> DiI and DAPI labeling of same field as shown in G. F> PGP9.5 labeling in the same field showing double labeling with the smaller DiI labeled axons on the detrusor muscles (arrowhead) and the larger DiI labeled nerve bundle shown in G and H (arrows). J> CG-NT animal showing DiI labeled smaller axons in association with detrusor muscle bundles (arrowheads). K> DiI and DAPI labeling of same field as shown in J. L> PGP9.5 labeling in the same field showing double labeling with the smaller DiI labeled axons on the detrusor muscles (arrowheads). Scale bars = 50 μm. All photomicrographs taken at same magnification. Abbreviations: GF-NT = genitofemoral nerve transfer, CG-NT = coccygeal nerve transfer; M=muscle.