Figure 3.
Higher power photomicrographs of detrusor muscle and intramural ganglia showing DiI or PGP9.5 labeled axons, and DAPI staining, a general nuclear stain in a GF-NT animal (A–E) and sham operated control animal (F–H). A> DiI labeling in a representative transverse histological section of detrusor muscle fibers (M as indicated in panel D) from a GF-NT animal. The detrusor muscle fibers are adjacent to a portion of an intramural ganglion (arrow; G as indicated in panel D). B> PGP9.5 labeling in the same field. C> Merged DiI and PGP.5. D> DAPI staining in the same field. E> Merged DAPI and DiI. F> DiI labeled axons in an intramural ganglia from a sham operated animal. G> DAPI staining in the same field. H> Merged DAPI and DiI. Scale bars = 50 μm.
