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Images of the opening of WT SHP2 taken from the PMF calculation at 30 top), 40 (middle) and 50 (bottom) Å using the time frame at the first 500 ps from the respective windows. The PTP (cyan), N-SH2 (blue) and C-SH2 (blue2) domains are shown in cartoon format. Residues shown include Cys459 (yellow), Asn92 (purple, on BG loop), Tyr66 (orange, on EF loop), Arg362 (green, on FH loop) and Asp425 (red, on LF loop).
