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. Author manuscript; available in PMC: 2012 Jun 1.
Published in final edited form as: Exp Parasitol. 2011 Feb 24;128(2):166–169. doi: 10.1016/j.exppara.2011.02.020

Figure 1.

Figure 1

Growth phenotypes of wild type, Δldodc, and knock-in parasites. L. donovani wild type (WT), Δldodc::NEO/Δldodc::HYG (Δldodc), and four independent knock-in clones (clones 1, 3, 4, and 5) were seeded at 1 × 105 parasites/ml in normal growth medium with either 200 μM putrescine (+), no additions (-), 50 μg/ml hygromycin (H), or 20 μg/ml neomycin (N). After five days of incubation at 28 °C, parasites were enumerated on a hemacytometer. The results are those of a single typical experiment that was performed in duplicate. Quantitation of parasite numbers employing Alamar Blue® as the growth indicator provided essentially identical results (data not shown).