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. Author manuscript; available in PMC: 2012 Jun 1.
Published in final edited form as: Exp Parasitol. 2011 Feb 24;128(2):166–169. doi: 10.1016/j.exppara.2011.02.020

Figure 2.

Figure 2

Southern and western blot analyses of knock-in parasites. (A) Genomic DNA from wild type (WT), Δldodc::NEO/Δldodc::HYG (Δldodc), and the four knock-in clones (clones 1, 3, 4, and 5) was digested with Sal I and hybridized separately to either LdODC, HYG, or NEO as indicated. Molecular weight markers are shown to the left. (B) Cell lysates from wild type (WT), Δldodc::NEO/Δldodc::HYG (Δldodc), and the four knock-in clones were fractionated by SDS polyacrylamide gel electrophoresis and probed with previously characterized rabbit polyclonal antibodies to LdODC and LdSPDSYN (as loading control). Molecular weight markers are indicated to the left.