Fig. 8.

In vitro effects of AG1478 and mAb 806. (A) Antiproliferative activity of AG1478 and mAb 806. A431 and U87MG.Δ2–7 cells were treated in vitro with AG1478 (6 μM) or mAb 806 (10 μg/ml) alone or in combination, and the data were expressed as percentage inhibition of cell growth. (Bars = SD.) (B) Effect of AG1478 treatment on antibody reactivity. A431 cells were treated for 10 min with AG1478 at the doses indicated, after which cells were lysed and the amount of EGFR was recognized by mAb 806 or 528 quantified by ELISA. Data are expressed as the percentage increase in antibody reactivity. (Bars = SD.)