Figure 3. Increased frequency of high-quality dual-cytokine producing effector cells in sirolimus-treated macaques.
RM were treated with sirolimus or left untreated during both vaccination and boost with MVA (n=4 in each group). PBMCs isolated at each timepoint were stimulated with vaccinia virus and assessed for IFN-γ and TNF by ICCS. Percentage of CD3+ CD8+ T cells which produce both TNF and IFN-γ at day 0 of vaccination with MVA and in the weeks following MVA are shown; up to three replicates for each RM at each timepoint are depicted. (* p<0.05, ** p<0.01).