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. Author manuscript; available in PMC: 2011 Jul 1.
Published in final edited form as: Nat Chem Biol. 2010 Nov 14;7(1):25–33. doi: 10.1038/nchembio.476

Fig. 5. Knocking-down sodium pump expression inhibits IFNβ induction.

Fig. 5

a. Efficient knock-down of ATP1a1 expression in 293T cells. For the full image, see Supplementary Figure 12c. b. SeV and dsDNA induced gene expression was inhibited in ATP1a1 knock-down cells. Control or ATP1a1 knock-down cells were infected with SeV or transfected with dsDNA for 6 hrs. RNA was harvested and Q-PCR conducted to monitor the expression of IFNβ and Cxcl10 genes. The expression levels were normalized to that of GAPDH gene. NT: Not treated. Data represent mean values ± s.d. (n=3). c–e. Knocking-down ATP1a1 expression in MEFs also reduced virus, dsRNA and dsDNA induced gene expression. MEFs with shRNA targeting ATP1a1 or a scramble sequence as control were subjected to SeV, poly I:C and poly dA:dT treatment. 6 hrs later, cells were harvested for either protein analysis (c, blot for Stat1, Trex1, ATP1a1 and β-actin proteins) or Q-PCR analysis (d–e) for the expression of IFNβ, Cxcl10, IRF7 (d), and Stat1, Trex1 and RIG-I (e) genes. The expression of β-actin gene was used as the reference. Data represent mean values ± s.d. (n=3). For the full image, see Supplementary Figure 12d.