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. Author manuscript; available in PMC: 2012 Apr 1.
Published in final edited form as: J Immunol. 2011 Feb 28;186(7):3977–3985. doi: 10.4049/jimmunol.1003588

FIGURE 4.

FIGURE 4

IFN-γ and IL-1β induces IL-27 mRNA in human monocyte derived macrophages. (A) Monocyte derived macrophages from normal donors (n = 7) were stimulated with rhIFN-γ or rhTNF-α or their combination or medium alone for 8 h prior to infection with L. donovani amastigotes. Macrophages were cultured for another 16 h before cells were lysed for mRNA expression analysis. (B) Monocyte derived macrophages from normal donors (n = 11) were stimulated with rhIFN-γ or rhIL-1β or their combination or medium alone for 24 h before cells were lysed for mRNA expression analysis. Significant differences are indicated with *, p < 0.05; **, p < 0.01; ***, p < 0.001.