| 30 μl (up to 5 ng) | Fragmented DNA sample |
| 15 μl | T4 DNA Ligase Buffer Master Mix (T4 DNA Ligase Buffer containing 10 mM ATP, 10 mM dNTP Mix) |
| 1 μl | Enzyme Master Mix (T4 DNA Polymerase, Klenow Enzyme, T4 PNK) |
| 4 μl | Water |
| 50 μl | Total Volume |
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