| 10 μl | 3′ End Modified DNA sample |
| 3.1 μl | T4 DNA Ligase Buffer 10X |
| 10 μl | Index PE Adapter Oligo Mix |
| 1 μl | T4 DNA Ligase |
| 6.9 μl | Sterile double-distilled water |
| 31 μl | Total Volume |
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