175 ng	Adapter-Ligated DNA sample
1 μl	PCR Primer InPE1.0 for fragment amplification
1 μl	PCR Primer InPE2.0 for fragment amplification
1 μl	PCR Index Primer
10 μl	5X HF Phusion Buffer
1 μl	10 mM dNTP
0.5 μl	Phusion DNA Polymerase
up to 50 μl	Sterile Double-Distilled Water
50 μl	Total Volume