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Detection of viral RNA in infected transgenic heart tissue by In situ hybridization analysis. (A) Nontransgenic heart tissue section 2 days after infection hybridized with digoxigenin-labeled (+) strand viral RNA. (B-D) Detection of (-) strand (B) and (+) strand (C and D) viral RNA in transgenic heart tissue sections at day 2 after infection. [Original magnifications, ×4(C) and ×40 (A, B, and D).]
