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. 2010 Aug 12;32(8):1025–1038. doi: 10.1093/eurheartj/ehq253

Figure 7.

Figure 7

4-HNE-protein adducts formation and differential phosphorylation of AMP-dependent protein kinase (AMPK)/Akt. (A) HNE-protein adducts formation evaluated with immunoblotting in hearts from wild-type (WT) and ALDH2 overexpression mice subjected to ischaemia or ischaemia/reperfusion (I/R). Inset: Representative gel blots depicting HNE-protein adducts formation. Molecular protein standards are shown on the left. The density of staining in each lane was assessed as a whole to generate a single value of the integrated density of all HNE-protein adducts formed within that lane; (B) HNE-protein adducts formation in hearts from WT and ALDH2 KO mice using immunoblotting; (C) In vivo regional ischaemia (20 min)-stimulated differential phosphorylation of AMPK in WT, ALDH2 overexpression, and ALDH2 KO mouse hearts. Inset: Representative gel blots depicting total and phosphorylation AMPK; and (D) in vivo regional ischaemia (20 min)/reperfusion (30 min)-stimulated differential phosphorylation of Akt in WT, ALDH2 overexpression, and ALDH2 KO mouse hearts. Inset: Representative gel blots depicting total and phosphorylation Akt. Mean ± SEM, n = 6–8, *P < 0.05 vs. sham or WT group under basal condition, #P < 0.05 vs. corresponding WT or ALDH2 group under ischaemia or I/R condition.