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. 2010 Aug 12;32(8):1025–1038. doi: 10.1093/eurheartj/ehq253

Figure 9.

Figure 9

Effect of 4-HNE on carbonyl formation, phosphorylation of LKB1 and PTEN. Cardiomyocytes from wild-type (WT) or ALDH2 overexpression mice were incubated with 4-HNE (20 µmol/L) or vehicle for 50 min prior to the assessment of carbonyl, total, and phosphorylated LKB1 and PTEN. (A) Carbonyl formation; (B) representative gel blots of total and phosphorylated LKB1 and PTEN (β-actin was used as loading control); (C and D) total LKB1 and PTEN expression; (E and F) phosphorylated LKB1 and PTEN; (G and H) phosphorylated-to-pan protein ratio of LKB1 and PTEN. Mean ± SEM, n = 5, *P < 0.05 vs. WT or control group, #P < 0.05 vs. WT + 4-NHE group.