Fig. 3.
Effect of NOEV after ganglioside loading on GM1 storage in cultured mouse fibroblasts expressing human β-galactosidase gene. The cells expressing mutant (R201C) or wild-type (GP8) human β-galactosidase were loaded with ganglioside mixture, and then cultured with additional 0.2 μM NOEV (4 days). Shown is staining with FITC-labeled cholera toxin B. (A and C) R201C cells. (B and D) GP8 cells. (A and B) After NOEV treatment. (C and D) Before NOEV treatment. GM1 storage was reduced to the control (GP8) level after NOEV treatment. There was no change in GP8 cells.