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. Author manuscript; available in PMC: 2012 Apr 1.
Published in final edited form as: Clin Cancer Res. 2011 Feb 24;17(7):1784–1795. doi: 10.1158/1078-0432.CCR-10-1891

Figure 2. BITC mediates nuclear retention of FOXO proteins in the tumors of BITC-treated mice.

Figure 2

Nuclear retention of FOXO proteins (A). Nuclear and cytosolic proteins from control and BITC 10μmol treated tumors were probed for FOXO proteins. Same membrane was stripped and reprobed for actin or lamin B to ensure equal protein loading. Concentration of BITC in the plasma and tumor tissues by LC/MS/MS (B). BITC concentration was evaluated in the plasma of the mice after 1h of oral gavage of 12μmol BITC, whereas cumulative concentration of BITC was determined in the tumors of mice after 46 days of BITC administration by LC/MS/MS. Bar represents the quantification of BITC in 10 samples (Mean ± SD).