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. Author manuscript; available in PMC: 2012 Mar 1.
Published in final edited form as: Wound Repair Regen. 2011 Mar–Apr;19(2):229–240. doi: 10.1111/j.1524-475X.2010.00655.x

Figure 3.

Figure 3

LPA-induced ERK1/2 phosphorylation is independent of EGFR. Serum-starved CCL-151 cells were stimulated with serum-free medium (control, CTL) or medium containing LPA (10 μM) in the absence or presence of inhibitors. Total cell lysates were immunoblotted with antibodies against phospho-ERK1/2 and total ERK1/2. A: CCL-151 cells were pretreated with Pertussis toxin (PTX) (100 ng/ml) for 18 hours, AG1478 (AG; 500 nM) for 1 hour, VPC32183 (VPC; 1 μM) for 30 minutes, GM6001 (GM; 10 μM) for 1 hour or vehicle (H2O for PTX; DMSO for AG1478 and GM6001; BSA for VPC32183) for the same period of time as each inhibitor, then stimulated with serum-free medium or LPA for 5 minutes (A) or for 1 hour (B). Results are representative of 3 independent experiments.