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. Author manuscript; available in PMC: 2011 Apr 14.
Published in final edited form as: J Immunol. 2010 Feb 3;184(5):2369–2376. doi: 10.4049/jimmunol.0902712

FIGURE 4. miRNA antagonists relieve native miRNA inhibition of IFNβ secretion.

FIGURE 4

Primary human macrophages were transfected with specific or control antagomiRs, chemically modifed to enhance stability and to hinder recognition by intracellular RNA sensors and subsequent activation of the interferon pathway. Macrophages were treated with poly I:C (50 ug/ml). After 24 hours, supernatants were collected and secreted IFNβ protein levels were measured by ELISA. Results from four independent experiments with macrophages from three donors are shown relative to IFNβ levels of poly I:C treated, no-miRNA controls; error bars indicate standard deviation.