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. Author manuscript; available in PMC: 2011 Apr 14.
Published in final edited form as: Microcirculation. 2009 May 26;16(6):508–520. doi: 10.1080/10739680902942271

Figure 3. Regions of venular convergences support increased leukocyte adhesion and TEM.

Figure 3

The number of rolling (A), firmly adhered (B), and leukocytes that underwent TEM (C) was quantified in straight venular regions (vessels 30–80μm diameter) and compared to regions of venular convergences (as defined in METHODS) in the same vessels under control and TNFα activated conditions. (D) The cartoon demonstrates the tissue area adjacent to venular convergence and straight venular region that was used to quantify leukocyte TEM. In venular convergences perpendicular lines were projected 40 μm away from the inner converging point. An equal area was used to define tissue regions adjacent to the straight venular region, while keeping the width of the ROI equal to the vessel diameter (gray arrows). Black arrow indicates the direction of blood flow. Following TNFα treatment a significantly higher number of adhered leukocytes accumulated in venular convergences, compared to straight venular regions and consequently more TEM was observed in these regions. * significantly different from each other (p<0.05), **/^ significantly different from each other (p<0.01), n=8 venules, 4 mice.