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. Author manuscript; available in PMC: 2012 Apr 1.
Published in final edited form as: J Neurochem. 2011 Mar 1;117(2):231–243. doi: 10.1111/j.1471-4159.2011.07194.x

Figure 4.

Figure 4

Effects of Na+ and TPEN on FluoZin-3 F488 (blue), Fura-2FF F340/F380 ratio (red), and the Fura-2FF Ca2+ isosbestic point, F356 (green). (a) Hippocampal neurons co-loaded with FluoZin-3 and Fura-2FF were exposed to Glu/Gly under Na+-free conditions (Na+ substituted with NMDG+) followed by an application of 158 mM Na+ and removal of NMDG+. The data are means ± standard error from 17 neurons monitored in a single experiment. The experiment was repeated 5 times with similar results. (b) The cells were treated as in (a) except that 10 μM TPEN was applied instead of Na+. The data are means ± standard error from 22 neurons monitored in a single experiment. The experiment was repeated 5 times with similar results.