Fig. 3.

Confirmation of the expression profiles. (A) cDNA prepared from steroid treated B. burgdorferi- (N40) infected NHPs and enriched by DECAL was used as template in PCRs by using gene-specific primers, as outlined in Materials and Methods. The amounts of template DNA from CNS and heart tissues in the PCR were normalized against the expression levels of flaB (bb0147). (B) Relative pixel intensities of the amplicons were measured by using scion image, an image processing and analysis software program (www.scioncorp.com).