Table 1. Strains and plasmids used in this study.
| Genotype/characteristics | Ref./Source | |
|---|---|---|
| Strain | ||
| Frag1 | F–rha thi gal lacZ | 47 |
| MJF372 | Frag1 rpoS::Tn10 | 48 |
| MJF405 | Frag1 clpP::Tn9 | 48 |
| MJF455 | Frag1 ΔmscS mscl::Cm | 11 |
| MJF507 | Frag1 ΔlacU169 | This study* |
| MJF513 | Frag1 ΔlacU169 rpoS::Tn10 | This study* |
| MJF530 | Frag1 ΔlacU169 ompR::Tn10 | This study* |
| MJF541 | Frag1 rssB::Tn10 | This study* |
| JM109 | endA1 recA1 gyrA96 thi hsdR17 [rK–,mK+] relA1 supE44 Δ[lac-proAB] [F′ traD36proAB lacl ZΔM15] | 49 |
| Plasmid | ||
| pMC1871 | pBR322 copy-number fusion vector | Amersham Pharmacia |
| pHSG575 | pSC101 copy-number cloning vector | 16 |
| pMCS | pMC1871 derivative carrying mscS promoter–lacZ cassette | This study |
| pMCL | pMC1871 derivative carrying mscL promoter–lacZ cassette | This study |
| pRLG770 | pBR322 derivative carrying transcription terminators | 17 |
| pRLG6984 | pRLG770 carrying the promoter region of mscL | This study |
| pRLG6985 | pRLG770 carrying the promoter region of mscS | This study |
| pHSGL | pHSG575 derivative carrying mscL promoter–lacZ cassette | This study |
| pHSGS | pHSG575 derivative carrying mscS promoter–lacZ cassette | This study |
| pHSGlacZ | pHSG575 derivative carrying promoter-less lacZ cassette from pMC1871 | This study |
*
Created by P1 transduction