Figure 2.

Tyk2 mRNA and protein expression is down regulated during acute SIV infection in brain. A. qRT-PCR analysis for tyk2 on RNA isolated from the brain of pigtailed macaques sacrificed between days 4 and 56 p.i. including uninfected controls. 5–6 animals used per group. Values are normalized to 18S ribosomal RNA and expressed as fold induction. Medians are connected by red dashes. * p=0.0649, ** p= 0.0022. B. Western blot analysis of tyk2 protein in lysates from brain of pigtailed macaques sacrificed between days 4 and 10 p.i. including uninfected controls. 6 animals were used per group. All are normalized to GAPDH and expressed as fold induction over uninfected. Medians are connected by red dashes. * p=0.0087, **p=0.0152, *** p=0.0022 C. qRT-PCR analysis for tyk2 on RNA isolated from the lungs of the same pigtailed macaques sacrificed between days 4 and 14 p.i including uninfected controls. Values normalized to 18S ribosomal RNA. Medians are connected by red dashes. *p=0.0317, # p=0.0635. D. Western blot analysis of tyk2 protein on lysates made from lung of pigtailed macaques sacrificed on day 4 p.i including uninfected controls. Values normalized to GAPDH and expressed as fold induction. Medians are indicated by red dashes. * p=0.0571. E. Western blot analysis of ptyk2 (Y1054/1055) and P-STAT3 (S727) on the same protein lysates, as well as a lysate made from pigtailed macrophages stimulated with interferon beta (100 units/mL) for 10 minutes (designated “+”).