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. 2011 Mar 29;108(15):6079–6084. doi: 10.1073/pnas.1011274108

Fig. 2.

Fig. 2.

Comparison of nucleotide incorporation for wtRNAP quenched with HCl or EDTA and Δ-loop RNAP quenched with EDTA. Plots of the percentages of complexes (normalized) that have incorporated CMP (i + 1) at template position +25 and AMP at template position +26 for wt and Δ-loop RNAP are shown. (A) CMP incorporation for the simultaneous addition experiments (100 μM CTP and 10 μM ATP) by wtRNAP quenched with HCl (black circles) or EDTA (blue squares) and Δ-loop RNAP quenched with EDTA (purple wedges). (B, C) CMP (B) and AMP (C) incorporation for the preincubation experiments (10 μM ATP prior to the addition of 100 μM CTP) by wtRNAP quenched with HCl (cyan diamonds) or EDTA (red triangles) and Δ-loop RNAP quenched with EDTA (purple wedges). Note that the scale for the x-axis in C is 0.2 s compared to 0.1 s for A and B. Error bars represent the standard deviation in the data. The curves are the best fits to single or double exponentials as determined by statistical F-test.