Fig. 1.

BAD is a unique substrate for PRMT1. (A) Several Akt target proteins were prepared as GST-fusion proteins and incubated with GST-PRMT1 in the presence of [³H]SAM. Reaction products were analyzed by autoradiography (Upper) and Coomassie brilliant blue staining (Lower). The arrowhead and asterisks indicate total amounts of GST-PRMT1 and GST-fused Akt target proteins, respectively. (B) HEK293T cells were transfected with expression vectors as indicated. Whole-cell lysates were immunoprecipitated (IP) with anti-FLAG antibody, followed by immunoblotting with anti-HA or anti-FLAG antibodies. (C) Whole cell lysates from HEK293T cells were immunoprecipitated with normal IgG or anti-BAD antibody, followed by immunoblotting with anti-PRMT1 or anti-BAD antibodies.