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. 2011 Mar 28;108(15):6085–6090. doi: 10.1073/pnas.1015328108

Fig. 4.

Fig. 4.

PRMT1 regulates binding of BAD to 14-3-3 and BCL-XL. (A) HEK293T cells were transfected with or without wild-type or G98R mutant of HA-PRMT1. Whole-cell lysates were immunoprecipitated with normal IgG or anti-BAD antibody, followed by immunoblotting with anti-14-3-3β or anti-BAD antibodies. (B) Whole-cell lysates from HEK293T cells transfected with control or PRMT1-specific siRNA were immunoprecipitated with anti-BAD antibody, followed by immunoblotting with anti-14-3-3β or anti-BAD antibodies. (C) HEK293T cells were transfected with control or PRMT1-specific siRNA together with empty or FLAG-BAD expression vectors. Whole-cell lysates were separated into cytoplasmic (C) and mitochondrial (M) fractions. Equal loading and verification of fractionation was confirmed by immunoblotting with anti-α-tubulin (cytoplasm) and anti-MnSOD (mitochondria) antibodies. (D) HEK293T cells were transfected with control or PRMT1-specific siRNA together with the HA-BCL-XL expression vector. Whole-cell lysates were immunoprecipitated with anti-BAD antibody, followed by immunoblotting as indicated.