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Rip2 up-regulation results in caspase-1 activation. Primary cortical neurons from wild-type mice cultured for 0, 2, 5, 7, 14, and 21 days were treated with 3 h OGD. (a) Cells were collected 6 h after OGD and lysates were immunoblotted with antibodies against Rip2, caspase-1, and β-actin. (Right) Densitometry using three samples for each condition. (b) Supernatants were assayed for LDH release.
