Fig. 4.

PHX-induced hepatic expression of FoxM1 is mediated by CB₁R via the Akt/PTEN pathway. Induction of Foxm1 mRNA assessed by real-time PCR (A) and FoxM1 protein documented by Western blotting (B) and immunohistochemistry (C) is blunted in the liver of CB₁R^−/− mice. (D) PHX increases p-Akt, decreases phosphorylated PTEN (p-PTEN), and increases the association of p-Akt with FoxM1 in livers from wild-type but not from CB₁R^−/− mice. p-Akt/FoxM1 complexes (bottom lane) were identified by immunoprecipitation with a FoxM1 antibody followed by Western blotting with a p-Akt antibody.