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. Author manuscript; available in PMC: 2012 Apr 22.
Published in final edited form as: Brain Res. 2011 Feb 19;1386:15–24. doi: 10.1016/j.brainres.2011.02.041

Figure 5. Effects of silencing vectors on cell viability cells human SH-SY5Y cells.

Figure 5

Cells were mock transfected or transfected with pAAV-H1-shRNA-Luc, pAAV-H1-shRNA-SNCA, pAAV-mir30-shRNA-NS or pAAV-mir30-shRNA-SNCA and harvested for Alamar Blue cell viability assays at 48 or 72 hrs post transfection. Graph shown is for one of two replicate experiments (n=3; mean ± SEM). Non-embedded shRNA vectors had the greatest effect on reducing cell viability. Cells transfected with pAAV-mir30-shRNA-NS did not show increased cell death. Note significantly reduced cell viability in cells transfected with pAAV-CMV-mir30shRNA compared to mock-transfected and the mir30-shRNA-NS control, but this was significantly less than in cells transfected with the non-embedded SNCA silencing vector; 2-way ANOVA (p<0.0001 for vector and time); Bonferonni posthoc tests: ***p≤0.001 for Mock vs H1shLuc, H1shSNCA and mir30shSNCA; H1shLuc vs H1shSNCA (72hrs), mir30shNS and mir30shSNCA; H1shSNCA vs mir30shSNCA; # p≤0.01 H1shLuc vs H1shSNCA (48hrs); NSD, Mock vs mir30shNS; H1shLuc vs mir30shSNCA.