Fig. 4.
Requirement for N- and C-terminal EF-hands for CDF. (a-d) CDF during 100-Hz trains of depolarizations as in Fig. 2 a-d [mean ± SEM for CaM (n = 11), CaM12 (n = 13), CaM34 (n = 9)], and CaM1234 (n = 7). (e) Averaged normalized current amplitudes between 0.5 and 1 s as in Fig. 2e. (f-i) CDF during paired-pulse protocols measured as in Fig. 2 e-i [mean ± SEM for CaM (n = 10), CaM12 (n = 6), CaM34 (n = 7), and CaM1234 (n = 5)]. (j) Mean values for P2max/P1max for ICa or IBa determined as in Fig. 2j. Asterisks indicate significant differences from WT (P < 0.05).