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. 2003 Dec 12;100(26):16059–16064. doi: 10.1073/pnas.2237000100

Fig. 5.

Fig. 5.

Binding of CaM to the IQ-like domain and the CBD. (a) His-tagged C-terminal fragments including the IQ-like domain (IM, amino acids 1848 - 1964), CBD (1959-2035), or an upstream sequence (1764-1848) used as a negative control. (b)Ca2+-dependent binding of His-tagged C-terminal fragments (His) to GST-tagged CaM (input, GST) immobilized on glutathione agarose in the presence of 2 μM Ca2+ or 10 mM EGTA was detected by immunoblot with anti-His antibodies. (c) Differential binding of CaM12 and CaM34 to the IQ-like domain and CBD. GST-tagged CaM12 or CaM34 was used to pull-down His-tagged α12.1 C-terminal fragments in 2 μMCa2+ as in b.(d) Model for CDI and CDF based on sequential interactions of the IQ-like domain and CBD with CaM.