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. Author manuscript; available in PMC: 2011 Apr 14.
Published in final edited form as: Cold Spring Harb Protoc. 2010 Oct 1;2010(10):pdb.prot5509. doi: 10.1101/pdb.prot5509
Reagent Quantity (for 50 ml) Final Concentration
MgCl2 250 μl 1M 5 mM
NaCl 5 ml 1M 100 mM
Tris (pH 9.5) 5 ml 1M 100 mM
Tween-20 50 μl 0.1%
Add dH20 to a total volume of 50 ml. Make just prior to use and store for no more than
three hours.
For color reactions, add 20 μl of NBT/BCIP solution (Roche Cat. #11681451001) to 1 ml
AP buffer and use immediately.