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. Author manuscript; available in PMC: 2011 Apr 14.
Published in final edited form as: Cold Spring Harb Protoc. 2010 Oct 1;2010(10):pdb.prot5509. doi: 10.1101/pdb.prot5509
Reagent Quantity (for 50 ml) Final concentration
SDS 5 ml 10% SDS 1.0%
Tween-20 250 μl 0.5%
Tris-HCl (pH 7.5) 5 ml 0.5 M Tris-HCl 50 mM
EDTA (pH 8.0) 100 μl 0.5 M EDTA 1 mM
NaCl 7.5 ml 1 M NaCl 150 mM
Add dH20 to a total volume of 50 ml and store at room temperature.